A mechanistic model improves off-target predictions and reveals the physical basis of SpCas9 fidelity
B. Eslami-Mossallam, M. Klein, C. v. d. Smagt, K. v. d. Sanden, S. K. Jones, J. A. Hawkins, I. J. Finkelstein, M. Depken
This paper develops a mechanistic model to predict SpCas9 off-target activity and explains its structural basis for fidelity.
bioRxiv · May 21, 2020 · PreprintBiophysics