Workflow for multiplex microsatellite panel development and sample preparation for robust amplicon sequencing of low-template and degraded DNA: validation for non-invasive genotyping in three large carnivore species
Marta De Barba, Frédéric Boyer, Molly Baur, Marjeta Konec, Elena Pazhenkova, Nadège Remollino, Céline Stoffel, Barbara Boljte, Christian Miquel, Tomaž Skrbinšek, Pierre Taberlet, Luca Fumagalli
The paper develops a workflow for designing and validating STR multiplex panels for HTS-based genotyping of low-template and degraded DNA, demonstrating high performance in three large carnivore species.
bioRxiv · Aug 21, 2026 · PreprintEcology