Measuring Illumina Size Bias Using REcount: A Novel Method for Highly Accurate Quantification of Engineered Genetic Constructs
Daryl M. Gohl, Alessandro Magli, John Garbe, Aaron Becker, Darrell M. Johnson, Shea Anderson, Benjamin Auch, Bradley Billstein, Elyse Froehling, Shana L. McDevitt, Kenneth B. Beckman
The paper introduces REcount, a PCR-free method for accurate quantification of engineered genetic constructs, showing it is highly accurate and reveals Illumina sequencing platform biases based on molecule length.
Genome biology · Apr 29, 2019Peer-reviewedGenomics