Cross-strain transferability of CRISPRi systems and design rules from laboratory to clinical Escherichia coli strains
Hyerim Ban, Stephen N. Rondthaler, Matthew Lebovich, Marcos A. Lora, Brandon Ugbesia, Lauren B. Andrews
The paper evaluates CRISPRi system performance across different E. coli strains and develops a dual gRNA system for improved repression in clinical isolates.
ACS Synthetic Biology · May 2, 2026Peer-reviewedSynthetic Biology