Importance of a heat snap in RT-PCR quantification of rotavirus double-stranded RNA in wastewater
Seju Kang, Anna Wettlauffer, Jolinda de Korne-Elenbaas, Charles Buregeya Niwagaba, Linda Strande, Dorothea H. Duong, Bridgette Shelden, Timothy Ryan Julian, Alexandria B B Boehm
This study shows that using a heat snap in RT-PCR increases rotavirus RNA quantification in environmental samples by 125-fold across different sample types and laboratories.
Environmental Science Water Research & Technology · Jan 1, 2025Peer-reviewedMolecular Biology