Live cell biosensors based on the fluorescence lifetime of environment-sensing dyes
B. Mehl, L. Li, E. Hinde, B. Liu, C. J. MacNevin, C.-W. Hsu, E. Gratton, K. M. Hahn
This study develops a FLIM biosensor using merocyanine dyes to detect conformational changes in Cdc42, showing improved sensitivity at low activation levels compared to FRET-based methods.
bioRxiv · Feb 8, 2022Cell Biology