K. M. Hahn

Actin nano-architecture of phagocytic podosomes

The paper uses advanced microscopy techniques to characterize the structure of phagocytic podosomes during frustrated phagocytosis, revealing their unique actin architecture and associated protein distributions.

Live cell biosensors based on the fluorescence lifetime of environment-sensing dyes

This study develops a FLIM biosensor using merocyanine dyes to detect conformational changes in Cdc42, showing improved sensitivity at low activation levels compared to FRET-based methods.

Mechanistic of Rac and Cdc42 synchronization at the cell edge by ARHGAP39-dependent signaling nodules and the impact on protrusion dynamics

This study identifies ARHGAP39's role in synchronizing Rac and Cdc42 activity at the cell edge through signaling nodules, impacting protrusion dynamics and cell invasion.

Correcting artifacts in ratiometric biosensor imaging; an improved approach for dividing noisy signals

The paper introduces a method to correct artifacts in ratiometric biosensor imaging by subtracting a noise correction factor from the numerator only, improving accuracy in low signal-to-noise regions.

Using SNAP-tag for facile construction of dye-based biosensors in living cells

The paper develops a biosensor using SNAP-tag to detect Cdc42 activation in living cells with ratiometric imaging capabilities.

Deep learning enables structured illumination microscopy with low light levels and enhanced speed

This paper uses deep learning to improve structured illumination microscopy, reducing image requirements and enabling low-light, high-speed super-resolution imaging.

Force-exerting lateral protrusions in fibroblastic cell contraction

The paper identifies force-exerting perpendicular lateral protrusions (PLPs) in fibroblasts under anisotropic fibrous environments, explaining desmoplastic expansion in cancer.

Combined Atomic Force Microscope and Volumetric Light Sheet System for Mechanobiology

The paper introduces a combined AFM and light sheet microscope for mechanobiology, enabling high-resolution imaging and force measurements of cellular processes.

VIEW-MOD: A Versatile Illumination Engine With a Modular Optical Design for Fluorescence Microscopy

The paper introduces a modular fluorescence microscope with flexible illumination and imaging modes, demonstrating its use in biosensor imaging, optogenetics, and 3D volume imaging.