Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and emulsion coupling assay as a low-cellularity workflow for single-cell cloning
T. Gross, C. Jeney, D. Halm, G. Finkenzeller, G. B. Stark, R. Zengerle, P. Koltay, S. Zimmermann
The paper introduces a low-cellularity workflow for characterizing CRISPR/Cas9 edited mesenchymal stem cells using single-cell printing and emulsion coupling assay to assess genetic and protein changes without large-scale clonal expansion.
PLOS ONE · Mar 4, 2021Peer-reviewedCell Biology