Temperature-dependence of Early Development of Zebrafish and the Consequences for Laboratory Use and Animal Welfare
Angelina Miller, Katja Schröder, Karsten Eike Braun, Caitlin Steindorf, Richard Ottermanns, Martina Roß-Nickoll, Henner Hollert, Thomas Backhaus
This study quantifies developmental differences in zebrafish embryos at 26°C and 28°C, showing temperature-dependent delays in key developmental endpoints and suggesting reevaluation of animal welfare guidelines.
PLoS ONE · Dec 31, 2025Peer-reviewedDevelopmental Biology