A Modular Toolkit For Theophylline-Dependent Synthetic Auxotrophs Via Riboswitch-Gated Essential Genes
Carlos Gonzalez-Lopez, Adrian Overly, Saahil Singh, Charlie Huang, Gabriel Lopez
The paper develops a modular platform for creating synthetic auxotrophs in E. coli using theophylline-responsive riboswitches, enabling reliable and low-cost biocontainment systems.
PLoS ONE · Oct 8, 2026Peer-reviewedSynthetic Biology