A general method to predict the effect of single amino acid substitutions on enzyme catalytic activity
Yu-Hsiu Tony Lin, Cheng Lai Victor Huang, Christina Ho, Maxim Shatsky, Jack F. Kirsch
The paper develops a machine learning method to predict which single amino acid substitutions in enzymes are likely to significantly reduce kcat/Km values, improving experimental efficiency.
bioRxiv · Dec 19, 2017 · PreprintBioinformatics