Advancing Luciferase Activity and Stability beyond Directed Evolution and Rational Design through Expert Guided Deep Learning
Spencer Gardiner, Joseph Talley, Tyler Green, Christopher Haynie, Corbyn Kubalek, Matthew Argyle, William P. Heaps, Joshua L. Ebbert, Deon Allen, Dallin Chipman, Bradley Charles Bundy, Dennis Della Corte
This paper develops enhanced NanoLuc luciferase variants using a hybrid approach of deep learning and structure-guided design, improving thermostability and activity at elevated temperatures.
ACS Catalysis · Jan 27, 2026Peer-reviewedBiochemistry