Measuring genome sizes using read-depth, k-mers, and flow cytometry: methodological comparisons in beetles (Coleoptera)
James M. Pflug, Valerie Renee Holmes, Crystal Burrus, J. Spencer Johnston, David R. Maddison
The paper compares genome size estimation methods using read-depth, k-mers, and flow cytometry in beetles, finding that no single method performs well across all species and that sequence-based methods tend to underestimate genome sizes.
G3 Genes Genomes Genetics · Jun 29, 2020Peer-reviewedGenomics