Amenability to Engineering of the Homologation Enzyme, HphA, through Homologous-Based Site-Directed Mutagenesis
Rebecca M. Lang Harman, Gracie Blackstone, Favor O. Aruna, Shivam R. Patel, Minho Shin, Reed K. NeSmith, D. Brooks Dickson, Angela C. Spencer, Shogo Mori
This study identifies key residues in HphA that influence substrate specificity through site-directed mutagenesis, showing that the A73L mutation broadens its substrate range.
bioRxiv · Dec 3, 2025 · PreprintBioengineering