High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display
A. Li, R. Voleti, M. Lee, D. Gagoski, N. H. Shah
This paper develops a platform to quantitatively profile sequence recognition by tyrosine kinases and SH2 domains using peptide libraries and deep sequencing, revealing phosphosite-specific mutations and binding affinities.
bioRxiv · Aug 1, 2022Biochemistry