Homology Directed Repair by Cas9:Donor Co-localization in Mammalian Cells
P. J. Roche, H. Gytz, F. Hussain, C. J. Cameron, D. Paquette, M. Blanchette, J. Dostie, B. Nagar, U. D. Akavia
The paper improves HDR efficiency in mammalian cells using Cas9 fused to a monoavidin domain and PEI delivery, achieving up to 90% HDR in three loci.
bioRxiv · Aug 6, 2018Biochemistry