High frame rate in vivo two-photon microscopy to quantify murine cerebrospinal fluid flow heterogeneity
Cooper Gray, Kaidi Hu, Dorothea Tse, Kyle Dieterle, Silas Simpson, Thomas Ruhl, Kyle Duff, Daehyun Kim, Mahsa Mirzaee, Anika Volker, Turki Saud Mohammed Alturki, Jeffrey Tithof
This study uses high frame rate two-photon microscopy to quantify spatial heterogeneity and pulsatility of CSF flow in mice, showing it is an emergent property of the hydraulic network.
bioRxiv · Sep 14, 2026 · PreprintNeuroscience