Design and optimisation of rapid real-time PCR assays for the detection of key Culicoides species
Elsie Isiye, Angela Valcarcel Olmeda, Thomas G. Curran, David O’Neill, Theo D. De Waal, Gerald Barry, Aidan O’Hanlon, James O’Shaughnessy, Marion England, Annetta Zintl, Denise B. O’Meara
This study develops real-time PCR assays for rapid detection of key Culicoides species, validated for specificity and efficiency in both single and mixed-species samples.
bioRxiv · Sep 26, 2025 · PreprintMolecular Biology