Design nonrepetitive and diverse activity single-guide RNA by deep learning
Yan Xia, Zeyu Liang, Xiaowen Du, Dengtian Cao, Jing Li, Lichao Sun, Yi‐Xin Huo, Shuyuan Guo
This study develops a deep learning model to generate nonrepetitive and diverse CRISPR sgRNAs with high activity, validated for gene editing and regulation in Bacillus subtilis.
bioRxiv · May 30, 2024 · PreprintSynthetic Biology