Synthetic BiologybioRxivPreprint
Design nonrepetitive and diverse activity single-guide RNA by deep learning
Yan Xia, Zeyu Liang, Xiaowen Du…·Shuyuan Guo
This study develops a deep learning model to generate nonrepetitive and diverse CRISPR sgRNAs with high activity, validated for gene editing and regulation in Bacillus subtilis.
DOI 10.1101/2024.05.30.596019