PAM-less Exonuclease-assisted Cas12a for visual detection of Vibrio Species
Derek Han Zhang, Siddharth Raykar, Kenneth Tsz Chun Ng
The paper introduces a PAM-less Cas12a-based assay for rapid, sensitive visual detection of Vibrio species without requiring a PAM site, using T7 exonuclease and low-cost materials.
bioRxiv · Oct 21, 2022 · PreprintSynthetic Biology