A simple, robust, broadly applicable insertion mutagenesis method to create random fluorescent protein - target protein fusions.
Andrew Pike, Cassandra Pietryski, Padraig Deighan, Jason N. Kuehner, Derek Lau, Anupama Seshan, Paul E. March
The paper describes a method to create random fluorescent protein-target protein fusions using transposition and screening, resulting in a useful library of functional chimeric proteins.
G3 Genes Genomes Genetics · Feb 16, 2024Peer-reviewedGenetics