FADS and semi-rational design modified T7 RNA polymerase reduced dsRNA production, with lower terminal transferase and RDRP activities
Qiongwei Tang, Sisi Zhu, Nannan Hu, Sainan Yin, Yuhong Yang, Yigang Teng, Dongliang Song, Xiang Liu
The paper evolves T7 RNA polymerase to reduce dsRNA production through FADS and saturated library screening, identifying variants with significantly lower dsRNA output and reduced immune stress.
bioRxiv · May 23, 2024 · PreprintMolecular Biology