Rationally designed mariner vectors to allow functional genomic analysis of Actinobacillus pleuropneumoniae and other bacteria by transposon-directed insertion-site sequencing (TraDIS)
Janine T Bossé, Yanwen Li, Leon G. Leanse, Liqing Zhou, Roy R Chaudhuri, Sarah E Peters, Jinhong Wang, Gareth A. Maglennon, Matthew TG Holden, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford, on behalf of the BRaDP1T consortium
This study develops two novel mariner vectors for TraDIS to identify conditionally essential genes in A. pleuropneumoniae and P. multocida, identifying 8 and 15 genes required for anaerobic growth.
bioRxiv · Oct 3, 2018 · PreprintMicrobiology