FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins
S. E. Hancock, E. Ding, E. Johansson Beves, T. W. Mitchell, N. Turner
This paper develops a high-throughput method for single-cell lipidomics that quantifies phosphatidylcholines and sphingomyelins using FACS, nanoESI, and shotgun lipidomics, enabling lineage distinction and subtle lipidome differences detection.
bioRxiv · Feb 15, 2022 · PreprintBiochemistry