T7 phage-assisted evolution of riboswitches using error-prone replication and dual selection
Eduardo Goicoechea Serrano, Carlos Blázquez-Bondia, Alfonso Jaramillo
This paper describes a dual-selection system using T7 phage to evolve functional theophylline riboswitches by alternating selection pressures in ON and OFF states.
Scientific Reports · Jan 29, 2024Peer-reviewedSynthetic Biology