P3 site-directed mutagenesis: An efficient method based on primer pairs with 3' overhangs
Negar Mousavi, Ethan Y. Zhou, Arezousadat Razavi, Elham Ebrahimi, Paulina Varela‐Castillo, Xiang‐Jiao Yang
The paper develops an optimized site-directed mutagenesis method using primer pairs with 3' overhangs, achieving higher efficiency and lower time requirements compared to existing methods.
Journal of Biological Chemistry · Jan 23, 2025Peer-reviewedMolecular Biology