Effects of duplicated mapped read PCR artifacts on RNA-seq differential expression analysis based on qRNA-seq
Anna C. Salzberg, Jiafen Hu, Elizabeth J. Conroy, Nancy M. Cladel, Robert M. Brucklacher, Georgina V. Bixler, Yuka Imamura Kawasawa
The paper evaluates the impact of PCR duplicates on RNA-seq differential expression analysis using qRNA-seq and finds that handling duplicates significantly improves sensitivity and specificity for single-end reads.
bioRxiv · Apr 13, 2018 · PreprintGenomics