Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus)
Lynsey R. Harper, Lori Lawson Handley, Christoph Hahn, Neil Boonham, Helen C. Rees, Kevin C. Gough, Erin Lewis, Ian P. Adams, Peter N. M. Brotherton, Susanna Phillips, Bernd Hänfling
This study compares eDNA metabarcoding and targeted qPCR for detecting the great crested newt, finding qPCR more sensitive but metabarcoding with no threshold comparable at a stringent qPCR threshold.
Ecology and Evolution · May 29, 2018Peer-reviewedMolecular Biology