Long Read Sequencing reveals transgene concatemerization and backbone integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes
M. W. Luqman, P. Jenjaroenpun, J. Spathos, N. Shingte, M. Cummins, P. Nimsamer, L. M. Ittner, T. Wongsurawat, F. Delerue
The paper uses long-read sequencing to analyze unintended on-target mutations in mouse zygotes edited with AAV-delivered CRISPR RNP complexes.
Frontiers in Genome Editing · Jun 4, 2025Peer-reviewedBioengineering