Construction and validation of EGFP-expressing Staphylococcus aureus clinical strains for adhesion and internalization assays on epithelial cells
S. Charaoui-Boukerzaza, M. F. Morgene, J. Rigaill, E. Audoux, Z. He, F. Grattard, A. Carricajo, B. Pozzetto, P. Berthelot, E. Botelho-Nevers, P. O. Verhoeven
The paper develops a one-step protocol to express EGFP in S. aureus clinical strains for studying adhesion and internalization in epithelial cells, showing high expression efficiency and similar behavior to wild-type strains.
bioRxiv · Mar 21, 2019 · PreprintMicrobiology