Random clonal expansion as a limiting factor in transplantable in vivo CRISPR/Cas9 screens
T. W. Lee, F. W. Hunter, W. R. Wilson, S. M. Jamieson
This study develops a transplantable in vivo CRISPR/Cas9 screen model for HNSCC, showing that gRNA representation declines due to clonal expansion, and identifies parameters to optimize screen success.
bioRxiv · Nov 28, 2021 · PreprintCancer Biology