Ultrafast pore-loop dynamics in a AAA+ machine point to a Brownian-ratchet mechanism for protein translocation
H. A. Mazal, M. Iljina, I. Riven, G. Haran
The paper measures pore-loop dynamics in ClpB using single-molecule FRET, finding microsecond fluctuations and conformational changes linked to substrate binding and disaggregation activity, suggesting a Brownian-ratchet mechanism.
bioRxiv · Nov 19, 2020Biophysics