Native ribonucleases process sgRNA transcripts to create catalytic Cas9/sgRNA complexes in planta
The paper shows that native ribonucleases process sgRNA transcripts in plants to create catalytically active Cas9/sgRNA complexes, challenging the current CRISPR/Cas9 dogma about 5' overhangs.
Plant Physiology · Jul 14, 2020Peer-reviewedPlant Biology