Engineered AAV Capsids with Enhanced Extracellular Vesicle Loading via Rational Design and Directed Evolution
Ioulia Vorobieva, Marina Luchner, Hans Friedrichsen, Ruhani Makkar, Simon Chester, Emily Haughton, Mariana Conceiao, Harrison Steel, Stephan J. Sanders, Matthew PA Wood, Dhanu Gupta
The paper engineers AAV9 capsids to enhance extracellular vesicle loading through rational design and directed evolution, achieving up to threefold increased EV-AAV yield and improved transduction efficiency.
bioRxiv · Dec 13, 2025 · PreprintSynthetic Biology