Dhanu Gupta

This study shows ML-assisted directed evolution improves AAV capsid manufacturing efficiency 41-fold compared to directed evolution alone.

This paper demonstrates efficient genome editing in non-human primate brains using engineered extracellular vesicles, achieving therapeutic potential for neurological disorders.

This study develops an engineered extracellular vesicle platform that improves shRNA loading and delivery efficiency, achieving robust gene silencing in multiple cell types and the mouse brain.

This study shows that systemic inflammation significantly prolongs EV plasma half-life and enhances their accumulation in inflamed organs like the liver and spleen.

This study investigates the role of tetraspanins in EV bioengineering by knocking out CD9, CD63, and CD81 in HEK293T cells and shows increased engineered EV production upon reintroduction of tetraspanin-fusion constructs.

The paper engineers AAV9 capsids to enhance extracellular vesicle loading through rational design and directed evolution, achieving up to threefold increased EV-AAV yield and improved transduction efficiency.

This study identifies uORF elements regulating BDNF translation and demonstrates that disrupting uORFs can upregulate BDNF expression in cell culture.

This paper develops hPep peptide-based nanoparticles for systemic mRNA delivery, showing effective tissue distribution and protein expression in mice and non-human primates.

This paper develops a method to isolate and characterize SMAPs from NK-92 cells, showing their cytotoxic activity against tumor cells in vitro and in vivo.

The paper identifies ThrEDs, a novel type of extracellular particle that accelerates wound healing and reduces fibrotic responses in patient-derived myofibroblasts.

This paper develops a PTTG1IP-based platform for efficient extracellular vesicle (EV) cargo delivery, showing improved therapeutic delivery compared to CD63-based scaffolds.

This study characterizes extracellular vesicles from activated CD4 T cells and shows their pro-inflammatory effects on antigen-presenting cells, providing a resource for EV-based therapeutic development.

This paper introduces a Snorkel-tag system for affinity purification of extracellular vesicles using CD81 fused with tags and transmembrane domains, enabling non-destructive isolation and future applications in tracing and analyzing EVs.

The paper identifies 24 novel scaffold proteins with conserved EV-sorting abilities, outperforming CD63 in cargo loading and demonstrating improved EV engineering potential.

This study engineers extracellular vesicles with IL6ST decoy receptors to inhibit the IL6 trans-signalling pathway, showing efficacy in vitro and in a Duchenne muscular dystrophy mouse model.