RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors
Mustafa Ahmad Munawar, Frank N. Martin, Anna Toljamo, Harri Kokko, Elina J. Oksanen
The paper introduces RPA-PCR couple to bypass DNA extraction in plant diagnostics, achieving specific detection of Phytophthora pathogens with sensitivity down to 10 fg of genomic DNA.
BioTechniques · Aug 20, 2020Peer-reviewedMolecular Biology