An integrated transcriptomics and proteomics study of Head and Neck Squamous Cell Carcinoma – methodological and analytical considerations.
Anupama Rajan Bhat, Manoj Kumar Gupta, Priya Krithivasan, Kunal Dhas, Jayalakshmi Nair, Ram Bhupal Reddy, Sudheendra HV, Sandip Chavan, Harsha Vardhan, Sujatha Darsi, Lavanya Balakrishnan, Shanmukh Katragadda, Vikram Kekatpure, Amritha Suresh, Pramila Tata, , Ravi Sirdeshmukh
This study compares two methods for RNA and protein extraction from tumor tissues, finding that sequential extraction improves the identification of differentially expressed transcripts and proteins and enhances pathway analysis.
bioRxiv · Aug 6, 2015Cancer Biology