Benchmarking Short-Read ITS2 and Full-Length ITS Sequencing Reveals Pipeline-Dependent Biases in Indoor Fungal Community Profiling
Mengyi Dong, A. Denene Blackwood, Megan E. J. Lott, Sherlynette Pérez Castro, Xavier Larkin, Thomas J. Clerkin, Heather Hemric, Jake Nash, Yeon Ji Kim, Jason Arnold, Lawrence A. David, Rytas J. Vilgalys, Anthony A. Fodor, Rachel T. Noble
This paper compares short-read ITS2 and long-read full-length ITS sequencing for fungal community profiling, finding that full-length ITS improves taxonomic resolution and ecological inference.
bioRxiv · May 15, 2026 · PreprintMicrobiology