Fragmentation of pooled PCR products for highly multiplexed TILLING
A. Tramontano, L. Jarc, J. Jankowicz-Cieslak, B. J. Hofinger, K. Gajek, M. Szurman-Zubrzycka, I. Szarejko, I. Inglebrecht, B. J. Till
This paper optimizes ultrasonication for fragmenting multiplexed PCR amplicons in TILLING to enable high-throughput mutation screening in pooled samples.
bioRxiv · Oct 24, 2018Plant Biology