PP271, a novel bacteriophage primase-polymerase, enables Primer-Free DNA Amplification
Alejandra Jimenez-Mejias, Susana Guerra-Gonzalez, Regina Olmos, Itziar Lazaro, Luis Blanco, Maria Isabel Martinez-Jimenez
This paper characterizes PP271, a novel bacteriophage PrimPol enzyme, showing it can synthesize DNA primers that are efficiently extended by Phi29 DNA polymerase for primer-free amplification.
bioRxiv · Nov 9, 2026 · PreprintBiochemistry