A sort-seq approach to the development of single fluorescent protein biosensors
J. N. Koberstein, M. L. Stewart, T. L. Mighell, C. B. Smith, M. S. Cohen
This paper introduces a sort-seq method to optimize single fluorescent protein biosensors by testing variants in parallel, identifying high-dynamic-range variants and improved linker sequences.
bioRxiv · Aug 21, 2020Biochemistry