DNA amplification in mixed aqueous-organic media: chemical analysis of leading polymerase chain reaction compositions
J. Neffati, I. Petrounia, R. D. Moreira, R. Chakrabarti
The paper identifies and quantifies co-solvents in PCR buffers using GC/MS to improve amplification of GC-rich regions by understanding their effects on PCR efficiency and specificity.
bioRxiv · Oct 2, 2021 · PreprintBiochemistry