Multiplexed gene editing with a multi-intron containing Cas9 gene in citrus
P. Sarkar, J. S. Vazquez, M. Zhou, A. Levy, Z. Mou, V. Orbovic
This paper introduces a multiplex gene editing system in citrus using a multi-intron Cas9 gene and gRNA-tRNA arrays to target the PDS gene, improving editing efficiency in Carrizo citrange and Duncan grapefruit.
bioRxiv · Dec 15, 2023Plant Biology