Ancestral reconstruction of polyethylene terephthalate degrading cutinases reveals a rugged and unexplored sequence-fitness landscape
Vanessa Vongsouthi, Rosemary Georgelin, Dana S. Matthews, Jake W. Saunders, Brendon M. Lee, Jennifer Ton, Adam M. Damry, Rebecca L. Frkic, Matthew A. Spence, Colin J. Jackson
This study uses ancestral sequence reconstruction to explore PET-degrading cutinase sequence space, revealing diverse evolutionary pathways and the effectiveness of LASE in mapping fitness landscapes.
Science Advances · May 14, 2025Peer-reviewedBiochemistry