Quantitative Substrate Kinetics Screening: One-Pot LC/MS Based Approach to Map Protease Specificity
Aaron Robinson, Garret D. Bland, Jessica Chu, James Ireland, Lilian Dao, Trinh Pham, Ming Dong, Vadim Demichev, Eric Johansen, Volker Schellenberger
This paper introduces a one-pot LC/MS-based method to quantitatively map protease substrate specificity using a large peptide library.
bioRxiv · Oct 6, 2025 · PreprintBiochemistry