Directed evolution expands CRISPR-Cas12a genome editing capacity
Enbo Ma, Kai Chen, Honglue Shi, Kevin M. Wasko, Isabel Esain-Garcia, Marena I. Trinidad, Kaihong Zhou, Jinjuan Ye, Jennifer A. Doudna
The paper identifies CRISPR-Cas12a variants with expanded PAM recognition, enabling targeting of ~25% of the human genome.
Nucleic Acids Research · Jul 8, 2025Peer-reviewedBiochemistry