Comparison of CRISPR and marker based methods for the engineering of phage T7
Aurelija Grigonyte, Christian Harrison, Paul R. MacDonald, Ariadna Montero‐Blay, Matthew Tridgett, John N. Duncan, Antonia P. Sagona, Chrystala Constantinidou, Alfonso Jaramillo, Andrew D. Millard
This study compares CRISPR and marker-based methods for engineering phage T7, finding marker-based selection most efficient and type II CRISPR-Cas easier to use than type I.
Viruses · Feb 10, 2020Peer-reviewedMolecular Biology