Several multiple sequence alignment perturbation methods enhance AlphaFold3 sampling of alternative protein states
Samuel Eriksson Lidbrink, Ivan Nissen, Jonathan Kenichi Ahrlind, Rebecca J. Howard, Erik R. Lindahl
The paper evaluates MSA perturbation methods on AlphaFold3 to improve sampling of alternative protein states, finding significant improvements in TM-scores compared to unperturbed AF3.
bioRxiv · Apr 3, 2026 · PreprintBiophysics