Seed sequences mediate off-target activity in the CRISPR-interference (CRISPRi) system
Neha Rohatgi, JP Fortin, Ted Lau, Yi Ying, Yue Zhang, Mike Costa, Rohit Reja
The paper investigates off-target activity in CRISPRi by analyzing a genome-wide sgRNA library and finds that seed sequences mediate most off-target effects.
bioRxiv · Apr 10, 2024 · PreprintBioinformatics